ABSTRACT
Objective:To investigate the expression and function of RIP3 in the liver of rats following ischemic postconditioning.Methods:The model of 70％ hepatic ischemia and reperfusion was established,then a total of forty healthy adult male Sprague-Dawley(SD) rats were divided randomly into four groups,ten rats in each group:a sham-operation group (Sham group);an ischemia reperfusion injury group(IR group);an ischemic postconditioning group(IPO group);an ischemic postconditioning and necrostatin-1 group (Nec-1 group).The blood samples and liver tissues were collected.The serum levels of ALT and AST were detected,and the liver histological examination was performed.Western-bolt was used to detect the TNF-α and RIP3 levels.Results:Compared with the IR group,ALT and AST in serum were significantly declined in the IPO group (P＜0.05);The liver damage after ischemia and reperfusion was improved obviously in the IPO group compared to which in the IR group;The Suzuki's scores was increased in the IR group compared to which in the IPO group (P＜0.05);There was a low grade of TNF-α and RIP3 in the Sham group,whereas the level of TNF-α and RIP3 significantly increased in the IR and IPO and Nec-1 group(P＜0.05);Compared with the IR group,the level of RIP3 was further decreased in the IPO group (P＜0.05);Compared with the IPO group,the level of RIP3 was further decreased in the Nec-1 group (P＜0.05).Conclusion:RIP3-mediated necroptosis was involved during hepatic ischemia postconditioning,and the protective effect of ischemia postconditioning may act as reducing necroptosis by cutting down the levels of RIP3.
ABSTRACT
Objective To evaluate the effects of non-wounded leg ischemic post-conditioning on the serum TNF-α and NO level of rats undergoing pancreas transplantation. Methods Group Sham consisted of 6 normal SD rats. 12 diabetic SD rats were randomly assigned to 2 groups: I/R group consisted of 6 diabetic rats which received ischemia reperfusion and NWLIPO group consisted of 6 diabetic rats which received ischemic post-conditioning. Blood glucose was measured before and after reperfusion. The level of serum NO and TNF-α was monitored 2 hours after long-time reperfusion. Results The level of blood glucose and TNF-α in NWLIPO group was lower than that in I/R group (P＜0. 01) while the level of NO was higher in NWLIPO group than in I/R group (P＜0.01). Conclusion Non-wounded leg ischemic post-conditioning can increase serum NO synthesis and down-regulate TNF-α.